ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of vascular endothelial growth factor (VEGF), CD44v6 in non-small cell lung carcinoma.</p><p><b>METHODS</b>The expression of VEGF and CD44v6 in 35 cases I approximately II stages, 27 cases III approximately IV stages and 50 cases with the metastasis of lymph nodes, 12 cases without metastasis of non-small cell lung carcinoma (NSCLC) tissues were studied by SP immunohistochemistry staining, and the 3 years survival rates were calculated in 42 cases patients.</p><p><b>RESULTS</b>The positive rates of VEGF and CD44v6 in NSCLC were 73% and 69%, respectively. They were both higher than those of matched normal lung tissues, P < 0.01. The expression of VEGF and CD44v6 were related to clinical stages and metastasis of lymph nodes significantly. The expression rates of the two markers in NSCLC with the metastasis of lymph nodes were higher than those without metastasis, chi(2) = 7.146 and 5.376 respectively, and those of III approximately IV stages were higher than those of I approximately II Stages, chi(2) = 6.392 and 12.152 respectively. Survival rates of three years were lower to those patients with positive expression of the two makers than those with negative expression. In a multivariate analysis, besides TNM stages and metastasis of lymph nodes, the positive expression of CD44v6 and the positive expression of VEGF were also the independent prognostic factors to affect the Survival time.</p><p><b>CONCLUSION</b>The VEGF and CD44v6 protein may act as one of important indexes to indicate the process of infiltration and metastasis in NSCLC.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Carcinoma, Non-Small-Cell Lung , Metabolism , Mortality , Follow-Up Studies , Hyaluronan Receptors , Metabolism , Lung Neoplasms , Metabolism , Mortality , Pathology , Lymph Nodes , Pathology , Lymphatic Metastasis , Neoplasm Staging , Proportional Hazards Models , Survival Rate , Vascular Endothelial Growth Factor A , MetabolismABSTRACT
Objective To investigate the significances of telomerase activity and P53 expression in Non-small cell lung cancer (NSCLC). Methods The activity of telomerase in cancerous tissues was detected by PCR-ELISA, and the expression of P53 protein was detected by S-P method. Results The telomerase activity and P53 expression were significantly higher in NSCLC tissues than in normal lung tissues. The telomerase activity and P53 expression had close association with the differentiation of cancer cells and lymph node metastasis. Conclusion It is very valuable to measure telomerase activity and P53 expression in studying the initiation and progression of NSCLC.
ABSTRACT
Objective To investigate the significances of telomerase activity and P53 expression in Non-small cell lung cancer (NSCLC). Methods The activity of telomerase in cancerous tissues was detected by PCR-ELISA, and the expression of P53 protein was detected by S-P method. Results The telomerase activity and P53 expression were significantly higher in NSCLC tissues than in normal lung tissues. The telomerase activity and P53 expression had close association with the differentiation of cancer cells and lymph node metastasis. Conclusion It is very valuable to measure telomerase activity and P53 expression in studying the initiation and progression of NSCLC.